Down-regulation of the alpha-2C adrenergic receptor: involvement of a serine/threonine motif in the third cytoplasmic loop

Table 1 Affinity (K_d) and density (B_max) of mutated alpha-2C adrenergic receptors expressed in CHO cells.

Clone	K_d, nM	B_max, fmol/mg protein	n
Wild-type (SSTS)	0.113 ± 0.008	372 ± 81	7
SSVA clone #1	0.105 ± 0.005	357 ± 103	3
SSVA clone #2	0.088 ± 0.009	90 ± 31	4
AAVA clone #1	0.118 ± 0.012	327 ± 105	3
AAVA clone #2	0.071 ± 0.009	39 ± 18	4

The K_d and B_max values are geometric and arithmetic means respectively from nonlinear regression analysis of saturation experiments with over a 100-fold concentration range of [³H]rauwolscine. Clone #1 and #2 were from separate transfections