Figure 3

Norepinephrine inhibition of [³H]rauwolscine binding. The data points are the means ± SEM of three independent inhibition experiments, each done in duplicate. The concentration of [³H]rauwolscine was 0.12 ± 0.01 pM. The IC₅₀ values determined from these data are 380 nM, 170 nM and 160 nM for the wild-type (SSTS), SSVA and AAVA receptors, respectively. The 100% values were 1223, 755 and 285 cpm for the wild-type (SSTS), SSVA and AAVA mutants, respectively. The means of the K_i values determined in the three individual experiments are given in Table 2.