Figure 6

Effects of clozapine i.t. or i.c.v. on the EMG recorded from the EUS during cystometry in anesthetized rats. Data from i.v. administration of clozapine [12] are presented for comparison. A) Clozapine i.t. had no effect on the integrated EMG recorded during phase 1 of the contraction. Only the highest dose of clozapine (50 nmoles) i.c.v. decreased the EMG during this phase to 62%; B) Clozapine i.t. or i.c.v. decreased the integrated EMG during phase 2 of the contraction. All doses of clozapine i.c.v. significantly reduced the EMG whereas only 5 and 50 nmoles i.t. resulted in significant reductions. C) Only the highest dose of clozapine i.c.v (50 nmoles) decreased the EMG during this phase of the contraction. Clozapine i.t. had no effects. D) The amplitude of the individual bursts of EMG occurring during phase 2 of the contraction were reduced by clozapine i.t and i.c.v. However, 5 nmoles of clozapine i.t. produced a reduction of EMG that was significantly different from the effects of the same dose given i.c.v. Only the highest dose (50 nmoles) was effective in reducing the EMG when administered i.c.v. V= vehicle administration (saline) for i.t. or i.c.v. groups; (V) = vehicle administration for i.v. group. ^* = p < 0.05; ^** = p < 0.01; # = p < 0.05 when compared to the effects of the same dose, different route.