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Figure 2 | BMC Pharmacology

Figure 2

From: Delta Opioid activation of the Mitogen-activated protein kinase cascade does not require transphosphorylation of Receptor Tyrosine Kinases

Figure 2

Western blots showing the effect of AG 1478 on ERK phosphorylation by EGF (2A) or DSLET (2B). (2A) HEK-δ-OR cells were serum-starved overnight prior to exposure to EGF (5 ng/ml for 5 minutes) in the presence or absence of PTX pretreatment (100 ng/ml; overnight) or AG1478 (1 μM; 60 minutes prior) . After DSLET exposure, cell lysates were prepared and ERK phosphorylation was determined by SDS PAGE, followed by immunoblotting of proteins onto nitrocellulose. Immunopositive bands correspond to α-tubulin, ERK 1, and ERK 2. (2B) AG1478 has no effect on DSLET-stimulated ERK phosphorylation. AG1478 (1 μM) was added 60 minutes prior to DSLET (1 pM – 1 μM for 5 minutes). After DSLET exposure, cell lysates were prepared and ERK phosphorylation was determined by SDS PAGE, followed by immunoblotting of proteins onto nitrocellulose. Immunopositive bands correspond to α-tubulin, ERK 1 and ERK 2. The immunoblots are representative of an experiment that was repeated three times.

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BMC Pharmacology

ISSN: 1471-2210