Immunofluorescent detection of lamin B in control (A, D, G) and treated (B, C, E, F, H, H') HCT-116 (B, C), A-549 (E, F) and MiAPaCa-2 cells (H, H'). Cells were grown on coverslips for 5-days without or with 1 × IC50 (B, E, H, H') or 10 × IC50 (C, F) RPR-130401. Cells were then fixed and incubated with an anti-lamin B monoclonal antibody, then with an Alexa®-conjugated secondary antibody. To visualize nuclei, the cells were counter-stained with DAPI (H'). Cells were viewed on a Zeiss microscope and photographed with Ilford HP-5 film. m, mitosis; p, cytoplasmic patches. The bar (B) represents 20 μm.