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Figure 4 | BMC Pharmacology

Figure 4

From: The farnesyl transferase inhibitor RPR-130401 does not alter radiation susceptibility in human tumor cells with a K-Ras mutation in spite of large changes in ploidy and lamin B distribution

Figure 4

Typical radiation survival curves for asynchronous growing cells without (•) or with exposure to 1 × IC50 () or 10 × IC50 (□) RPR-130401. Cells were plated, exposed to the drug for 3-days and irradiated. Each flask was trypsinized immediately after irradiation and 800 to 1,000 cells were replated in drug-free medium for colony formation assays. To account for the loss of plating efficiency du to drug exposure, survival values were normalized relative to the surviving fraction for drug alone. The curves were drawn for best fit to a linear quadratic equation (eq. (2), see Materials and Method) taking all data points into consideration. Found: α = 0.518 ± 0.036 Gy-1, β = 0.044 ± 0.016 Gy-2 for HCT-116 cells; α = 0.193 ± 0.036 Gy-1, β = 0.065 ± 0.014 Gy-2 for A-549 cells; α = 0.189 ± 0.058 Gy-1, β = 0.086 ± 0.026 Gy-2 for MiAPaCa-2 cells. Each data point came from an average over three flasks or more. Bars, SD. The figure at bottom right shows the surviving fraction at 2 Gy (SF2) of HCT-116 cells in the presence of RPR-130401. The conditions were the same as above. Data were corrected for the expression of potentially lethal damage [82].

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