Figure 2
Immunoprecipitation with anti-Flag M2 affinity resin of solubilized HEK-293 cells co-expressing nNOS and HF-tagged α1B- (Flag-B/nNOS) or α1D-ARs (Flag-D/nNOS) or HEK-293 cells expressing only the HF-tagged α1-AR subtypes (Flag-B, Flag-D). A: Western blot with anti-Flag antibody; arrows indicate monomers, dimers and trimers of the HF-tagged α1B- (closed) and α1D- (open) ARs. B: Western blot with anti-nNOS antibody. Blots shown are representative of at least two other experiments with similar results. IP = immunoprecipitation; IB = immunoblot.