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Figure 1 | BMC Pharmacology

Figure 1

From: Interaction of neuronal nitric oxide synthase with alpha1-adrenergic receptor subtypes in transfected HEK-293 cells

Figure 1

Immunoprecipitation with anti-Flag M2 affinity resin of solubilized HEK-293 cells co-expressing nNOS and HF-tagged α1A-AR (Flag-A/nNOS) or HEK-293 expressing only nNOS (HEK/nNOS). Cells were solubilized, immunoprecipitated with anti-Flag M2 affinity resin, eluted with Flag peptide, run on SDS-PAGE, and transferred to nitrocellulose as described in Methods. A: Western blot with M2 anti-Flag antibody with arrows showing monomers, dimers and trimers; B: Western blot with anti-nNOS antibody. Blots are representative of at least two other experiments with similar results. IP = immunoprecipitation; IB = immunoblot.

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BMC Pharmacology

ISSN: 1471-2210