Figure 8

RT-PCR analysis demonstrating distribution of EP2 receptor (right upper panel) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (right lower panel) mRNA along rabbit nephron. PCR products were electrophoretically separated on a 1% agarose gel. RNA loading was corrected by normalizing to GAPDH mRNA expression. Data are from a single experiment and represent three independent experiments. Left panels showing positive and negative control for EP2 (upper) and GAPDH (lower). Note: PCT, proximal convoluted tubules; PST, proximal straight tubules; tDL, the thin descending limb of Henle; TAL, thick ascending limb; DCT, distal convoluted tubules; CCD and MCD, cortical and medullary collecting ducts.