Figure 2

Competition binding profile of the EP₂ receptor expressed in COS-1 cells. Membranes were incubated with 1.5–2 nM [³H]PGE₂ and varying concentrations of competitors. The data shown are from a single experiment performed in triplicate and are representative of three to four experiments. Panel A: ■ PGE₂; ▼ PGF_2a; ▲ PGD₂; ◆ iloprost. Panel B: □ butaprost free acid; ?? AH13205; ▽ misoprostol; ◇ sulprostone.