Table 1 Rates of the transitions observed in kinetics.
Transition | Time constants | Transition rates |
|---|---|---|
Bimolecular NO binding to 5c-His | τB = 0.25 ms; [NO]= 20 µM | kB = 2 x 108 M-1s-1 |
Conversion 6c-NO → 5c-NO | τ5C2 = 43 ms | k 5C 2 = 23 s-1 |
Geminate NO rebinding to 5c-His | Ï„G2 = 6.5 ns | kG2 = 0.15 x 109 s-1 |
Conversion 6c*-NO → 5c*-NO | τ5C1 = 0.66 µs | k5C1 = 1.5 x 106 s-1 |
His rebinding to 4c-heme | Ï„His = 62 ps | kHis = 1.4 x 1010 s-1 |
Geminate NO rebinding to 4c-heme | Ï„G1 = 7 ps | kG1 = 0.13 x 1012 s-1 |
Structural relaxation sGC* → sGC | 0.66 µs < τR < 250 µs | 4 x 103 s-1 <kR <1.5 x 106 s-1 |