Protection of protease-activated receptor 2 mediated vasodilatation against angiotensin II-induced vascular dysfunction in mice

Table 2 Acetylcholine concentration-response curves of mesenteric arteries from saline- and angiotensin II-treated mice.

Treatment ⁱ	Strain	n	-log EC₅₀ (M)	E_max (%)	Hill slope	n	-log EC₅₀ (M) ^a	E_max (%) ^{b c}	Hill slope
	Saline				Angiotensin II
Controls	C57	47	7.5(0.1)	90(1)	1.0(0.1)	52	7.0(0.1)	78(3)^d	1.0(0.1)
	PAR2^-/-	14	7.7(0.1)	91(2)	1.0(0.1)	13	7.5(0.3)	77(5)	0.9(0.2)
L-NAME	C57	13	7.5(0.2)	63(5)^{e f}	0.7(0.1)	19	6.8(0.3)	49(5)^{g h}	0.7(0.1)
	PAR2^-/-	8	7.2(0.3)	67(8)^{e f}	0.7(0.1)	6	7.4(0.3)	68(13)	1.1(0.2)
indomethacin	C57	8	7.4(0.2)	96(2)	1.5(0.6)	14	7.3(0.2)	81(4)	0.8(0.1)
	PAR2^-/-	8	7.5(0.3)	87(3)	1.0(0.1)	7	7.6(0.1)	76(8)	0.9(0.2)
L-NAME + indomethacin	C57	12	7.3(0.3)	70(6)^{e f}	0.8(0.2)	15	7.6(0.4)	59(6)^{g h}	0.6(0.1)
	PAR2^-/-	7	7.7(0.2)	69(10)^e	0.7(0.1)	5	7.4(0.2)	66(8)	0.6(0.1)
FR122047	C57	7	7.5(0.1)	87(3)	1.1(0.1)	11	6.9(0.3)	71(6)	1.5(0.8)
NS398	C57	8	7.7(0.3)	89(3)	1.3(0.3)	10	7.2(0.3)	78(8)	0.7(0.1)
FR122047 + NS398	C57	3	7.5(0.3)	75(4)	1.0(0.5)	7	7.4(0.2)	74(8)	1.3(0.3)
AH6809 + L798106 + L161982	C57	6	7.5(0.2)	90(3)	1.2(0.2)	6	6.8(0.2)	91(3)	1.2(0.3)
CAY10441	C57	7	7.4(0.2)	88(3)	1.0(0.1)	7	6.8(0.3)	86(3)	1.1(0.3)

Values are mean (SE), n = number of mice. Variables were determined by curve fitting data points from cumulative drug concentration-responses relationships to a four parameter logistic function. Treatments of arteries included antagonists of COX-1 (1 μM FR122047), COX-2 (0.3, 3 μM NS398), COX-1/2 (10 μM indomethacin), NOS (100 μM L-NAME), prostaglandin E₂receptors (1 μM AH6809, 1 μM L798106, 0.1 μM L161982), prostaglandin I₂receptor (0.1 μM CAY10441). Comparisons were made by two-way ANOVA (pump × artery treatment) [^aP < 0.05, ^bP < 0.0001, effect of pump (saline vs. angiotensin II), ^cP < 0.001, effect of artery treatments] followed by Bonferroni post hoc tests: ^dP < 0.05, ANG II C57 controls compared to saline C57 controls; ^eP < 0.05, inhibitor group compared with saline C57 controls; ^fP < 0.05, inhibitor group compared with saline PAR2^-/- controls; ^gP < 0.05, group compared with ANG II C57 controls; ^hP < 0.05, inhibitor group compared with ANG II PAR2^-/- controls; ⁱ data from PAR2^-/- were included as artery treatment factor for analyses.
C57, C57BL/6J; E_max, maximum relaxation response where 100% is complete reversal of contraction; PAR2^-/-, protease-activated receptor 2 gene knockout mice.