Figure 3
Acetylcholine-induced vasodilatations in C57 and PAR2-/- mice administered saline and ANG II. C57BL/6J (C57) and protease-activated receptor 2 gene knockout (PAR2-/-) mice were pretreated with saline or angiotensin II (ANG II) for two weeks prior to experiments. Values are the mean ± SE (n = number of mice) for ACh-induced relaxations of second order mesenteric arteries contracted submaximally by cirazoline. Arteries were exposed to inhibitors [100 μM L-NAME, 10 μM indomethacin, 100 μM L-NAME + 10 μM indomethacin] for 20 min prior to cirazoline. *P < 0.05, saline vs. ANG II in (A, C) -log EC50 and Emax and (B) Emax. **P < 0.05, inhibitor treatment compared with controls (A, untreated) within same pump treatment. Comparisons by two-way ANOVA (inhibitor treatments × pump) followed by Bonferroni post hoc test.