Figure 9
Effect of CCl 4 with or without prior administration of natansnin on Bax and Bid. Mitochondrial protein extracted from control, CCl4 and natansnin (10 mg/kg, 20 mg/kg body weight) treated rats and separated on 10% SDS gels and transferred on to nitrocellulose membranes. Immunoblots were detected with Bax and Bid specific primary antibodies. The expression levels of β-actin were used as loading controls. Densitometric analysis showing the protein levels of Bax and Bid were increased in CCl4 group while treatment with natansnin significantly decreased the protein levels. Data are represented as mean ± standard deviation from three independent experiments (*p < 0.05 indicate significant difference relative to the CCl4 treated rats).