Figure 1

Top: Blot of 5-HT-induced stimulation of the ERK/MAPK pathway in rat aortic vascular smooth muscle cells as measured by use of a phosphospecific ERK/MAPK antibody. Densitometry measurements are for the 42 kDa band. Blot is representative of four experiments. Bottom: Total ERK/MAPK antibody recognizes nonphosphorylated forms as well as phosphorylated protein. Densitometry measurements are for the 42 kDa band.