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Figure 8 | BMC Pharmacology

Figure 8

From: Effect of a short-term in vitro exposure to the marine toxin domoic acid on viability, tumor necrosis factor-alpha, matrix metalloproteinase-9 and superoxide anion release by rat neonatal microglia

Figure 8

The effect of LPS and Domoic acid on neonatal rat brain microglia O 2 - release. O2- generation was determined as described in Materials and Methods. (A) Neonatal rat brain microglia (250,000 cells/well) were pretreated with LPS [10 ng/mL] and/ or Domoic acid [1 mM] for 6 hours and then stimulated with PMA [1 μM] for 2 hours. (B) Neonatal rat brain microglia (250,000 cells/well) were first pretreated with LPS [10 ng/mL] for 6 hours and then stimulated with PMA [1 μM] ± Domoic acid [0–1 mM] for 2 hours. Data (O2- nanomoles/2 hours) are expressed as mean ± SD of values obtained from three culture wells of one representative experiment. **p < 0.01 vs. untreated control.

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BMC Pharmacology

ISSN: 1471-2210