The time-dependent effect of LPS and Domoic acid on neonatal rat brain microglia TNF-α release. Neonatal rat brain microglia (2.8–5 × 106 cells/culture dish) were treated with Domoic acid [1 mM] or LPS [10 ng/mL] for 1–6 hours. TNF-α was determined as described under Materials and Methods. Data (pg/mL) are expressed as mean ± SE of 2 independent experiments. **p < 0.01 vs. vehicle control.