Skip to main content

Table 3 Sequence of the marked CNBr fragments in figures 5 and 6. MW: molecular weight; AA: amino acid; the several amino acids are shown in the one-letter-code. Sequenced fragments of the α1-subunit are signed as in Tab. 4, while CNBr fragments of the β1-subunit are defined by the position of the determined AA in the subunit. The CNBr-2 fragment of the α1-subunit, represented in all labeled bands,

From: NO-independent regulatory site of direct sGC stimulators like YC-1 and BAY 41-2272

CNBr-fragment α1-subunit β1-subunit Sequence from the N-terminus MW [kDa]
CNBr I CNBr -1 to 8   APGQVPTEPIEE... 51.8
CNBr II CNBr -1 to 4   APGQVPTEPIEE... 35.9
CNBr III CNBr -1 and 2   APGQVPTEPIEE... 30.2
CNBr IV CNBr 16   PRYCLFGNNVT... 11.2
  CNBr 8   IYIVESSAILFLG... 12.5
  CNBr -2 and 3   PPCFRSECTEF... 11.7
   AA 505-619 EIAGQVQVDGE... 12.8
CNBr V CNBr -10 to 13   LNALYTRFDQQ... 6.5
  CNBr 3   LDRDLAILQLG... 5.5
  CNBr 2   PPCFRSECTEF... 6.2
   AA 2-69 YGFVNHALELL... 7.7
   AA 116-164 RAPSFRCTDAE... 5.5
   AA 445-503 KIVNLLNDLYTR... 6.7
   AA 538-592 PRYCLFGNTVN... 6.2
CNBr VI CNBr -2 to 6   PPCFRSECTEF... 14.7
  CNBr 16   PRYCLFGNNVT... 11.2
   AA 505-599 EIAGQVQVDGE... 10.4
CNBr VII CNBr -2 and 3   PPCFRSECTEF... 11.7
  CNBr 8   IYIVESSAILFLG... 12.5
CNBr VIII CNBr 2   PPCFRSECTEF... 6.2
   AA 445-480 KIVNLLNDLYTR... 4.3
   AA 2-69 YGFVNHALELL... 7.7
   AA 1-69 MYGFVNHALEL... 7.7