Table 3 Sequence of the marked CNBr fragments in figures 5 and 6. MW: molecular weight; AA: amino acid; the several amino acids are shown in the one-letter-code. Sequenced fragments of the α1-subunit are signed as in Tab. 4, while CNBr fragments of the β1-subunit are defined by the position of the determined AA in the subunit. The CNBr-2 fragment of the α1-subunit, represented in all labeled bands,
From: NO-independent regulatory site of direct sGC stimulators like YC-1 and BAY 41-2272
CNBr-fragment | α1-subunit | β1-subunit | Sequence from the N-terminus | MW [kDa] |
|---|---|---|---|---|
CNBr I | CNBr -1 to 8 | APGQVPTEPIEE... | 51.8 | |
CNBr II | CNBr -1 to 4 | APGQVPTEPIEE... | 35.9 | |
CNBr III | CNBr -1 and 2 | APGQVPTEPIEE... | 30.2 | |
CNBr IV | CNBr 16 | PRYCLFGNNVT... | 11.2 | |
CNBr 8 | IYIVESSAILFLG... | 12.5 | ||
CNBr -2 and 3 | PPCFRSECTEF... | 11.7 | ||
AA 505-619 | EIAGQVQVDGE... | 12.8 | ||
CNBr V | CNBr -10 to 13 | LNALYTRFDQQ... | 6.5 | |
CNBr 3 | LDRDLAILQLG... | 5.5 | ||
CNBr 2 | PPCFRSECTEF... | 6.2 | ||
AA 2-69 | YGFVNHALELL... | 7.7 | ||
AA 116-164 | RAPSFRCTDAE... | 5.5 | ||
AA 445-503 | KIVNLLNDLYTR... | 6.7 | ||
AA 538-592 | PRYCLFGNTVN... | 6.2 | ||
CNBr VI | CNBr -2 to 6 | PPCFRSECTEF... | 14.7 | |
CNBr 16 | PRYCLFGNNVT... | 11.2 | ||
AA 505-599 | EIAGQVQVDGE... | 10.4 | ||
CNBr VII | CNBr -2 and 3 | PPCFRSECTEF... | 11.7 | |
CNBr 8 | IYIVESSAILFLG... | 12.5 | ||
CNBr VIII | CNBr 2 | PPCFRSECTEF... | 6.2 | |
AA 445-480 | KIVNLLNDLYTR... | 4.3 | ||
AA 2-69 | YGFVNHALELL... | 7.7 | ||
AA 1-69 | MYGFVNHALEL... | 7.7 |