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Table 1 Stimulation of purified sGC in the presence and absence of DTT by the NO donor SNP. YC-1 and the ortho-. meta- and para-PALs with Mg2+ as cofactor. Shown are the x-fold stimulations versus basal specific activity of SNP (1 μM). YC-1 (100 μM, 10 μM, 1 μM and 1 μM YC-1 in combination with 1μM SNP) and the different PALs (100 μM, 10 μM, 1 μM and 1 μM PAL in combination with 1 μM SNP). The data presented represent the mean ± SEM out of four independent experiments performed in duplicate.

From: NO-independent regulatory site of direct sGC stimulators like YC-1 and BAY 41-2272

Stimulation of purified sGC [fold increase vs. basal activity ± SEM] (basal specific activity [nmol/mg/min])
Stimulator Concentration + DTT -DTT
Basal   1.0 ± 0.1 (64 ± 8) 1.0 ± 0.2 (150.8 ± 33.4)
SNP 1 μM 82.3 ± 10.5 17.1 ± 2.2
  100 μM 45.9 ± 7.7 44.4 ± 4.8
YC-1 10 μM 4.5 ± 0.8 7.0 ± 1.7
  1 μ M 2.0 ± 0.4 1.5 ± 0.2
YC-1 + SNP 1 μM + 1 μM 98.4 ± 17.3 20.6 ± 3.6
  100 μM 14.4 ± 1.6 21.4 ± 4.2
ortho-PAL 10 μM 2.9 ± 1.5 3.1 ± 0.6
  1 μ M 2.1 ± 1.0 1.0 ± 0.1
ortho-PAL + SNP 1 μM + 1 μM 85.7 ± 11.4 15.8 ± 2.0
  100 μM 54.7 ± 10.2 56.1 ± 0.1
meta-PAL 10 μM 21.3 ± 7.1 23.3 ± 4.1
  1 μ M 4.5 ± 1.0 4.9 ± 0.4
meta-PAL + SNP 1 μM + 1 μM 185.6 ± 72.6 39.0 ± 5.6
  100 μM 36.6 ± 3.5 30.2 ± 4.5
para-PAL 10 μM 15.3 ± 2.7 17.3 ± 1.9
  1 μM 3.5 ± 0.4 3.8 ± 0.3
para-PAL + SNP 1 μM + 1 μM 229.1 ± 33.6 50.6 ±5.5