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Figure 7 | BMC Pharmacology

Figure 7

From: NO-independent regulatory site of direct sGC stimulators like YC-1 and BAY 41-2272

Figure 7

Coomassie-blue stained Western Blot and autoradiogram of photoaffinity labeled sGC fragments after CNBr digest, SDS-PAGE on a TRIS-Tricin-gel (16.5%) (48 h exposition on Imaging-Plate screened by a folio of contamination monitors). Shown are a 14C-labeled rainbow molecular weightmarker (14C-M), further molecular weightmarkers, and photoaffinity labeled sGC (200 μg) after CNBr digest (sGC CNBr). The arrows show the molecular weightmarkers with their molecular weights (kDa) or the 3H-meta-PAL labeled sGC fragments (CNBr VI-VIII) with their molecular weights, as calculated after sequencing.

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