Figure 6

Coomassie-blue stained Western Blot and autoradiogram of photoaffinity labeled sGC fragments after CNBr digest and SDS-PAGE on a 10–20% gradient gel (6 h exposition on Imaging-Plate). Shown are a ¹⁴C-labeled rainbow molecular weightmarker (¹⁴C-M), further molecular weightmarker, photoaffinity labeled sGC (200 μg) after CNBr digest (sGC CNBr) and for control undigested sGC (sGC; 5 μg). The arrows show the molecular weightmarkers with their molecular weights (kDa) or the ³H-meta-PAL labeled sGC fragments (BrCN I-V) with their molecular weights, as calculated after sequencing.