Figure 4

Dose-dependent effect of 25-OH-cholesterol on c-jun activation. Cells were treated with 0. 1.25, 2.5, 5, 10 or 20 μM 25-OH-cholesterol for 12 hours, then processed for Western blot analyses. Antibodies against c-jun, phospho-c-jun (at serine 63) and ERK 1/2 were used in this set of experiments. Concurrent electrophoresis procedures were performed for each antibody. Results indicated that 25-OH-cholesterol caused a dose-dependent increase in c-jun activation.