- Poster presentation
- Open access
- Published:
Opposite effects of cGMP-dependent protein kinase on cGMP production by soluble and particular guanylyl cyclase in adult ventricular myocytes
BMC Pharmacology volume 7, Article number: P10 (2007)
Background
Both particulate (pGC) and soluble guanylyl cyclases (sGC) synthesize cGMP. While sGC is activated by nitric oxide (NO), pGC is activated by natriuretic peptides (NPs) such as ANP or BNP. Physiological effects of cGMP are mainly mediated by cGMP-dependent protein kinase (PKG) and cGMP phosphodiesterases (PDEs), namely PDE2 and PDE5. We hypothesised that PKG may underlie regulatory feedback in cGMP signals produced by pGC and sGC activation in adult rat ventricular myocytes (ARVMs).
Materials and methods
To test this hypothesis, subsarcolemmal cGMP signals elicited by NO-donors or NPs in the presence of the PKG inhibitor, KT5823 (KT, 50 nM) were monitored in ARVMs. Myocytes were infected by an adenovirus expressing the WT rat olfactory cyclic nucleotide-gated channel CNGA2 and the cGMP-gated current (ICNG) was recorded by the whole-cell patch-clamp technique.
Results
Application of the membrane permeant cGMP analog Sp-8-pCPT-cGMPS (Sp-8, 100 μM) induced a large ICNG current in myocytes infected by CNGA2 adenovirus, but not in control cells. KT increased 3-fold the ICNG current followed by activation of sGC by S-nitroso-N-acetyl-penicillamine (SNAP, 100 μM). This effect was abolished in the presence of the non selective PDE inhibitor IBMX (100 μM) or a selective PDE5 inhibitor (sildenafil, 100 nM). Surprisingly, KT decreased (by 50%) the ICNG current stimulated by ANP (10 nM and 100 nM) in a PDE-independent manner, but had no effect on total cGMP content measured by radioimmunoassay, which is controlled by PDE2 and PDE5 subtypes.
Conclusion
Our results suggest that in ARVMs the subsarcolemmal cGMP pools generated by sGC or pGC are differentially regulated by PKG. In the sGC pool, PDE5 mediates the effects of PKG, while in the pGC pool, the PKG effect is independent of PDE activity. These differential regulations of cGMP signals may underlie the specific effects of NPs and NO-donors on cardiac function.
Author information
Authors and Affiliations
Rights and permissions
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Castro, L., Fischmeister, R. Opposite effects of cGMP-dependent protein kinase on cGMP production by soluble and particular guanylyl cyclase in adult ventricular myocytes. BMC Pharmacol 7 (Suppl 1), P10 (2007). https://doi.org/10.1186/1471-2210-7-S1-P10
Published:
DOI: https://doi.org/10.1186/1471-2210-7-S1-P10