Figure 2
From: Structural and functional definition of the specificity of a novel caspase-3 inhibitor, Ac-DNLD-CHO
Hydrolysis of Ac-DNLD-MCA by caspases. The time course of the abilities of caspases-3 (A), -7 (B), -8, (C) and -9 (D) to cleave the fluorometric caspase substrates Ac-DNLD-MCA (●) and Ac-DEVD-CHO (○) were compared. The cleavage assay was performed as described in "Methods" The y-axis represents the concentration of MCA production (pmol) and the x-axis represents incubation period. Data indicate the mean of three independent experiments.