Gene expression profiling reveals effects of Cimicifuga racemosa (L.) NUTT. (black cohosh) on the estrogen receptor positive human breast cancer cell line MCF-7

Table 1 Primers used for real-time RT-PCR

Gene	Primer Sequence 5'-3'		Product Size
Baculoviral IAP repeat-containing 5 (BIRC5)	for	AAAGCATTCGTCCGGTTG	152 bp
	rev	CCGCAGTTTCCTCAAATTCT
Cyclin E2 (CCNE2)	for	GACTGCTGCTGCCTTGTG	151 bp
	rev	AAAAGTCTTCAGCTTCACTGGA
Cyclin G2 (CCNG2)	for	CCCAGAACCTCCACAACAG	158 bp
	rev	GGTGCACTCTTGATCACTGG
Cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1)	for	TCTTCGCTACCTACCCAACC	196 bp
	rev	ATCTGACAGCTGGACATTGG
Cytochrome P450, family 1, subfamily B, polypeptide 1 (CYP1B1)	for	AGAACGTACCGGCCACTATC	175 bp
	rev	GGCTGGTCACCCATACAAG
DNA-damage-inducible transcript 4 (DDIT4)	for	GTTTGACCGCTCCACGAG	166 bp
	rev	CATCAGGTTGGCACACAAGT
DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9)	for	GGATGCTGAAGCAAAATTCA	150 bp
	rev	AATGACTGCTCAAAAGAACTTCC
E2F transcription factor 2 (E2F2)	for	CGCATCTATGACATCACCAAC	157 bp
	rev	TGTTCATCAGCTCCTTCAGC
Estrogen receptor, alpha (ESR1)	for	CAGACACTTTGATCCACCTGA	179 bp
	rev	CTCCAGCAGCAGGTCATAGA
GREB1 protein (GREB1)	for	ATCATCCTGAACGTGGACCT	151 bp
	rev	CCACGATCTGCTTCTTCATC
Growth arrest and DNA-damage-inducible, alpha (GADD45A)	for	GGAGGAAGTGCTCAGCAAA	169 bp
	rev	CTGGATCAGGGTGAAGTGG
Metastasis associated in lung adenocarcinoma transcript 1 (MALAT-1)	for	TGCAATTTGGTGATGAAGGT	161 bp
	rev	CAACATATTGCCGACCTCAC
Proliferating cell nuclear antigen (PCNA)	for	TTGCACTGAGGTACCTGAACTT	160 bp
	rev	CCTTCTTCATCCTCGATCTTG
Vascular endothelial growth factor (VEGF)	for	CATCTTCAAGCCATCCTGTG	179 bp
	rev	TGCATTCACATTTGTTGTGC
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	for	ACCAGGTGGTCTCCTCTGAC	173 bp
	rev	TTACTCCTTGGAGGCCATGT