Figure 2From: Reduced inhibitory action of a GABAB receptor agonist on [3H]-dopamine release from rat ventral tegmental area in vitro after chronic nicotine administrationCalcium-dependence and tetrodotoxin (TTX) sensitivity of the electrically evoked release of [3H]-DA from ventral tegmental area slices. Electrical stimulation (20 mA, 2 Hz, 4 min) occurred 14 min (S1) and 59 min (S2) after the beginning of sample collection. Slices were perfused with buffer alone (control), calcium-free medium containing 1 mM EGTA (Ca2+-free), or buffer containing 1 μM TTX for 15 min before and during S1 (black bar). After 18 min from the beginning of sample collection, all samples were superfused with normal buffer. Values represent mean ± s.e.mean (n = 3 rats).Back to article page