In vivo models of lung neutrophil activation. Comparison of mice and hamsters

BMC Pharmacology

Table 1 Levels of bronchoalveolar lavage myeloperoxidase (MPO) activity, elastase activity and TNF-α following LPS challenge in BALB/c mice.

	TNF-α 3 h (ng/ml)	MPO 3 h (mU/ml)	MPO 24 h (mU/ml)	Elastase 24 h (ng/ml)
PBS (50 μl)	0 ± 0	5.1 ± 4.8	56.7 ± 7.2	0 ± 0
LPS (0.05 mg/kg)	4.5 ± 0.6^*	4.5 ± 2.4	733.3 ± 103.4^*	18.3 ± 1.23 ^*
LPS (0.1 mg/kg)	15.3 ± 1.2^*	14.1 ± 8.3	961.8 ± 320.4^*	62.5 ± 3.5^*
LPS (0.3 mg/kg)	24.5 ± 1.45^*	35.4 ± 5.2^*	1209.8 ± 170.4 ^*	252.1 ± 18.5^*

Animals were intranasally challenged with increasing concentrations of LPS (0.05 to 0.3 mg/kg) or PBS alone (50 μl). Three or 24 hours after the challenge, animals were sacrificed and bronchoalveolar lavage performed. Data are expressed as mean ± SEM from two different experiments each included 6 mice per group. Significance, (P < 0.05) indicated by (*) is versus PBS-challenged mice